Topfluor cholesterol
WebTopFluor-labeled lipids are great aids in studying the distribution of lipids, lipid diffusion rates, and lipid dynamics. TopFluor has been used to study accessibility in the lipid … WebFor technical questions email us at [email protected] People do amazing things with our lipids. What will you do?
Topfluor cholesterol
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WebA total of 20 nmol ml −1 TopFluor-cholesterol complexed with 0.1% MCD in DMEM containing 1.5% LDS was loaded to the cells for 10 min on ice. After rinsing with PBS, cells were incubated at 37 ... Web23- (dipyrrometheneboron difluoride)-24-norcholesterol (TopFluor Cholesterol). 878557-19-8. Catalog No. NC0746020. $366.92 / Each of 1. Qty Check Availability. Add to cart. …
Web31. dec 2012 · Cholesterol plays an important role in determining the biophysical properties of biological membranes, and its concentration is tightly controlled by homeostatic … WebTopFluor® Derivatives. Sterols. BUY 810255 - TopFluor® Cholesterol; BUY 810288 - 16:0 TopFluor® cholesterol; BUY 810290 - 18:2 TopFluor® cholesterol; BUY 810299 - 25-(C4 …
Web9. júl 2024 · Cells were treated with TopFluor Cholesterol and BODIPY C12 for 48 h to allow incorporation in LDs. Cells were then incubated in Mitotracker Deep Red for 45 min to label mitochondria (Table 1 ). WebIn the first part of this protocol, we describe how to synthesize and image CTL in living cells relative to caveolin, a structural component of caveolae. In the second part, we explain in detail how to perform time-lapse experiments of commercially available BODIPY-tagged cholesterol (TopFluor-cholesterol ®; TF-Chol) in comparison to DHE ...
WebCell-based reporter gene assay demonstrated that compound 1 showed higher potency by lipid depletion treatment. Collectively, our findings indicate that eliminating cholesterol …
WebCells were treated with MCD, permeabilized by digitonin, and then incubated with 16:0 TopFluor® cholesterol. Mitochondria were stained with Mitotracker-Red. (G) Pearson's Co-localization Coefficient of Mitotracker-Red and 16:0 TopFluor® cholesterol in F. n = 20. ∗∗∗p 0.001 by student's t-test. reine catherine d\u0027aragonWeb1. jún 2024 · As expected, the IC 50 value of TO901317 was right-ward shifted depending on increasing TopFluor cholesterol concentration . It is difficult to increase TopFluor cholesterol concentration more than 10 μM (about twofold higher than K d) because of its solubility. These data demonstrate that TO901317 inhibited TopFluor cholesterol binding … reine carthageWebOne such analog with minimized alteration of cholesterol's properties is the commercially available TopFluor- cholesterol (i.e., cholesterol tagged with the BODIPY fluorophore at carbon 24, Fig. 1 ... reine catherine d\u0027angleterreWebHere, we describe new cationic analogues of BODIPY-Cholesterol (TopFluor-Cholesterol, TF-Chol), which combine a positive charge on the sterol side chain and a BODIPY group connected via a C-4 linker. In contrast to TF-Chol, the new analogues TF-1 and TF-3 possessing acetyl groups on the A ring (C-3 position on steroid) internalized much faster ... procure to pay accounting roleWeb1. dec 2024 · Here, we describe new cationic analogues of BODIPY-Cholesterol (TopFluor-Cholesterol, TF-Chol), which combine a positive charge on the sterol side chain and a … reineck apothekeWeb12. aug 2024 · A S. cerevisiae expressing PTC-3 or PTC-3 D697A were incubated for 2 h with 5 mM TopFluor® Cholesterol, washed, re-suspended in cholesterol-free buffer, and after 20 min, fluorescence intensity ... reinecke ballade orchestra youtubeWeb16:0 TopFluor ® cholesterol carries saturated acyl chains. Approximately 30-40% of the mammalian plasma membrane consists of cholesterol. TopFluor ® has a moiety, called … procure that 意味