Phospholipidosis assay

Author: yirx

August undefined, 2024

WebinstaCELL® Phospholipidosis Assay Kits. Drug induced accumulation of phospholipids (phospholipidosis) is associated with clinical toxicities. Cell‐based assays can help to … WebMar 1, 2024 · This assay has been optimized and validated in HepG2 and HepRG cells, and miniaturized into a 1536-well plate, thus can be used for high-throughput screening (HTS) to identify chemical compounds...

Liver organoids: established tools for disease modeling and drug ...

WebSteatosis is increased lipid accumulation, mainly as triglycerides, in the liver, while phospholipidosis is a lysosomal storage disorder characterised by intracellular … WebJul 2, 2008 · In vitro screening assays were developed in HepG2 cells, rat primary hepatocytes, and rhesus monkey hepatocytes using the fluorescent-labeled phospholipid probe N - (7-nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl- sn -glycero-3-phosphoethanolamine (NBD-PE) or Nile Red lipid stain. thai restaurants in hilton head island

Cell-Based Imaging Assay for Detection of Phospholipidosis

WebIn this study, a cell-based phospholipidosis assay has been developed using the LipidTOX Red reagent in HepG2 cells and miniaturized into a 1536-well plate format. To validate this assay for high-throughput screening (HTS), the LOPAC library of 1280 compounds was screened using a quantitative HTS platform. A group of known phospholipidosis ... WebPhospholipidosis is a lysosomal storage disorder characterized by the excess accumulation of phospholipids in tissues, often stimulated by drugs. Drug induced hepatotoxicity is a leading cause of attrition during drug development. WebTraductions en contexte de "monitored by assay" en anglais-français avec Reverso Context : If self-injection with Improvac is suspected, reproductive physiology should be monitored by assay of testosterone or oestrogen levels (as appropriate). The risk thai restaurants in hayes kent

Detection of Phospholipidosis Induction: A Cell-Based Assay in …

WebPhospholipidosis (PL) is lysosomal storage disorder characterized by the excessive accumulation of intracellular phospholipids (Figure 1). The mechanism of phospholipidosis is still unclear today, but it is known that phospholipidosis is commonly caused by the intake of a special group of drugs containing a hydrophobic ring/chain and a hydrophilic amine … WebJun 29, 2012 · The presence or absence of phospholipidosis in the in vitro assay correlated well with ultrastructural findings and drug accumulation in rat tissues. ABT-770, which produced phospholipidosis ... synonyme für coachingWebNational Center for Biotechnology Information thai restaurants in hayward ca

"WebMar 5, 2015 · Synthesis and phospholipidosis effect of a series of cationic amphiphilic compounds: a case study to evaluate in silico and in vitro assays Article Full-text available " - Phospholipidosis assay

Phospholipidosis assay

Drug-Induced Phospholipidosis and Steatosis Assay

WebMar 24, 2024 · Drug-induced phospholipidosis: Human: ASCs: Matrigel: Expression of CYP450 enzyme (CYP3A4); ... (LoT) assay to analyze the hepatoxicity of 238 marketed drugs (32 negative controls and 206 reported DILI agents) and showed high predictive values of the LoT system with 88.7% sensitivity and 88.9% specificity. WebPhospholipidosis (PL) is a phospholipid storage disorder, resulting in excessive accumulation of phospholipids in lysosomes of various tissue types [199, 200]. From:Progress in Medicinal Chemistry, 2011 Related terms: Amiodarone Patient Phospholipid Storage Disease Lysosome Storage Disease Niemann Pick Disease …

Did you know?

WebPhospholipidosis (PLD) is a lysosomal storage disorder induced by compounds, notably cationic amphiphilic drugs, which although reversible interferes with cellular … WebTo identify compounds that may induce phospholipidosis early in the discovery process, we have developed a predictive fluorescent cell-based assay amenable to automated high …

WebJun 21, 2024 · 1. Introduction. Drug-induced phospholipidosis (DIPLD) is characterized by excessive intracellular accumulation of phospholipids as a result of drug treatments [1].Since the first DIPLD case reported in 1948 [2], an increasing number of drugs were identified as PLD inducers, including antibiotics, antidepressants, antipsychotics and so … WebFeb 1, 2007 · Phospholipidosis was determined by algorithms that evaluated the granular texture of the perinuclear area as an indicator of the amount of phospholipidosis vacuoles. ... High-content screening...

WebCytotoxicity Assays. Cytotoxicity is the degree to which a substance can cause damage to a cell. A substance or process that causes cell damage or death is referred to as cytotoxic, "cyto" meaning cell and "toxic" meaning poison. Cells exposed to cytotoxic compounds may undergo necrosis (uncontrolled cell death), apoptosis (programmed cell ... WebIn this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H 2 …

Webcontent phospholipidosis assay using the LipidTOX reagent in a high-throughput screening (HTS) plat- form. This assay has been optimized and validated in HepG2 and HepRG cells, and miniaturized into a

WebSuch assays rely heavily on the development prospective assays aim to predict toxicities that could lead to the ter- of human physiologically relevant model systems in combination with mination of a drug candidate or an entire development programme, appropriate toxicity end points. and bespoke retrospective assays are used for issue resolution ... synonyme für complianceWeb+ selectively discriminates phospholipidosis from steatosis assay ready HepG2 cells assay ready HepG2 cells have an instantly high viability, low debris and attach within 24 hours. drug induced phospholipidosis assay ready HepG2 were treated for 48 hours with Sertraline, a potent inductor of phospholi-pidosis, and Cyclosporine A for negative ... thai restaurants in hiram gaWebDec 1, 2011 · In summary, we demonstrated an in vitro phospholipidosis screening assay in CHO-K1 and HepG2 cells based on a high content screening analysis method that can … thai restaurants in holden maWebFeb 1, 2006 · To investigate the role of phospholipidosis in antiviral activity in vitro, we tested 19 drugs for their induction of this effect in A549 cells using the well-established NBD-PE staining assay... synonyme für clubWebSep 3, 2013 · In this study, a cell-based phospholipidosis assay has been developed using the LipidTOX Red reagent in HepG2 cells and miniaturized into a 1536-well plate format. … synonyme für experimentWebWhen studying phospholipidosis/steatosis in vitro, IONTOX uses HepG2 cells in combination with LipidTox Red, as our primary model to discriminate between phospholipidosis … thai restaurants in hollywood caWebMar 1, 2024 · The EM method, the gold standard of detecting cellular phospholipidosis, has downsides, namely, low-throughput, high-costs, and unsuitability for screening a large … synonyme für famous